FastQCFastQC Report
Fri 10 Feb 2017
ERR1147916.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147916.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3899490
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178330.4573162131458216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108540.2783440911503812No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90670.23251758563299302No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68350.060.02671
GTATCAA127950.056.778421
TCAACGC171450.042.0814934
AACGCAG172100.041.8188746
CAACGCA175200.041.180785
ATCAACG175650.041.0414163
TATCAAC187300.038.7824132
ACGCAGA200950.035.903827
CGCAGAG209050.034.5411158
GCAGAGT226900.031.9286589
GTACATG194950.029.9952141
TACATGG203500.028.620372
ACATGGG201850.028.4358633
AGAGTAC212600.027.6974510-11
GAGTACT100300.026.3299412-13
CAGAGTA221650.025.57366810-11
AGTACTT105300.025.16443612-13
CATGGGG114600.024.8091074
GTACTTT111100.023.79718414-15
GTAAGTA21750.022.9761352
Produced by FastQC (version 0.11.5)