FastQCFastQC Report
Fri 10 Feb 2017
ERR1147915.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147915.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3557106
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165240.464534933735458No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97040.2728060395163934No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81830.2300465603217897No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49850.063.6819421
GTATCAA104100.062.4778671
TCAACGC143150.045.291984
CAACGCA145450.044.575785
ATCAACG145900.044.275843
AACGCAG147750.043.8013576
TATCAAC160150.040.869992
ACGCAGA172850.037.440857
CGCAGAG178850.036.151548
GCAGAGT195400.033.1504559
GTACATG166450.030.3435461
TACATGG172300.029.1816772
ACATGGG170200.028.6929973
AGAGTAC182550.028.6087510-11
GAGTACT91400.025.47837612-13
CAGAGTA189450.025.4160710-11
CATGGGG95850.025.0712184
CATGGGT46100.024.9024774
AGTACTT96150.024.5908812-13
AGTACAT127050.022.70666112-13
Produced by FastQC (version 0.11.5)