FastQCFastQC Report
Fri 10 Feb 2017
ERR1147914.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147914.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2155080
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70500.3271340275070995No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53070.24625535942981233No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37400.17354344154277335No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26100.075.3088151
GTATCAA50950.056.93211
TCAACGC67900.042.309194
CAACGCA70200.041.0924455
ATCAACG71050.040.433413
AACGCAG73950.038.8477826
TATCAAC81500.035.7681582
ACGCAGA85250.033.768237
CGCAGAG89150.032.290998
GCAGAGT97700.029.586879
GTACATG82700.028.5208241
TACATGG85650.027.0891482
AGAGTAC90900.026.50015610-11
ACATGGG87200.026.3968323
GAGTACT45450.025.5186712-13
CAGAGTA95350.025.4505310-11
GTACTTT48550.023.7667514-15
AGTACTT47000.023.2217812-13
CATGGGT31400.022.3516564
GTATAGA5600.022.3359131
Produced by FastQC (version 0.11.5)