FastQCFastQC Report
Fri 10 Feb 2017
ERR1147913.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147913.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2882506
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122260.42414482398302034No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73750.2558537605819381No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61480.21328663322817018No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43300.065.3408051
GTATCAA88050.061.7618371
TCAACGC119850.045.0664834
CAACGCA121450.044.5217485
ATCAACG121750.044.4128153
AACGCAG123900.043.7854046
TATCAAC129850.041.8364872
ACGCAGA142850.038.0186237
CGCAGAG150350.036.1616828
GCAGAGT161150.033.7750979
GTACATG137700.032.095841
ACATGGG140400.030.8444943
TACATGG142900.030.6042352
AGAGTAC151900.028.46967310-11
CATGGGG85300.027.8943844
GAGTACT75350.027.63062112-13
AGTACTT79500.026.56238612-13
CAGAGTA156300.026.39327810-11
GTACTTT80000.026.06176814-15
ATGGGGG44200.023.0133445
Produced by FastQC (version 0.11.5)