FastQCFastQC Report
Fri 10 Feb 2017
ERR1147912.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147912.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3849303
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT230490.5987837278593033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132020.34297118205555654No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114540.29756036352555254No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA150550.065.2330551
GGTATCA67750.064.347191
TCAACGC207450.047.135894
ATCAACG208900.046.8371933
CAACGCA211700.046.1896135
AACGCAG213250.046.0212336
TATCAAC218650.044.815842
ACGCAGA247600.039.5405357
CGCAGAG254300.038.451998
GCAGAGT271350.036.1454969
GTACATG248000.032.1315961
TACATGG258800.030.8054812
ACATGGG254400.030.7915823
TAGATCG2351.0913936E-1130.3722345
AGAGTAC261750.029.60881810-11
CATGGGG144400.028.6273374
AGTACTT125200.026.91280212-13
ATGGGGG74900.026.602745
GAGTACT119350.026.53753312-13
CAGAGTA267200.026.46724510-11
Produced by FastQC (version 0.11.5)