FastQCFastQC Report
Fri 10 Feb 2017
ERR1147911.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147911.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3617206
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223780.6186542873145737No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126600.3499938903120254No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108910.3010887408679517No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73950.060.9686051
GTATCAA153500.060.4903761
TATCAAC208400.044.595112
TCAACGC208800.044.3264544
ATCAACG212100.043.6087463
AACGCAG213350.043.3253676
CAACGCA213850.043.307515
ACGCAGA246950.037.4546057
CGCAGAG251900.036.789448
GCAGAGT269950.034.3515789
GTACATG244950.031.7794721
TACATGG250850.030.78682
ACATGGG250450.030.3761983
CATGGGG154600.029.8947834
AGAGTAC261150.028.32302510-11
GAGTACT116000.027.76670312-13
AGTACTT123200.027.1578712-13
ATGGGGG80600.027.0103325
ATGGGAG27650.026.4602435
GTAAGTA28350.026.2337822
Produced by FastQC (version 0.11.5)