FastQCFastQC Report
Fri 10 Feb 2017
ERR1147910.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147910.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4764375
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152820.3207556080283353No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91070.19114784205693297No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75010.15743932834841926No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59000.064.6035841
GTATCAA119100.059.8064581
TCAACGC160000.044.0906074
CAACGCA163050.043.265855
ATCAACG166250.042.4330643
AACGCAG167350.042.154156
TATCAAC179200.039.712712
ACGCAGA194100.036.3446547
CGCAGAG203500.034.753528
GCAGAGT222050.031.9841589
GTACATG195350.028.2309991
AGAGTAC203450.027.87689210-11
TACATGG203350.026.9495682
ACATGGG201300.026.4460373
CAGAGTA211100.025.75368310-11
GAGTACT95650.025.4964812-13
GTACTTT104550.023.43983514-15
AGTACTT103400.023.26908712-13
CATGGGG117500.022.577634
AGTACAT153900.021.41176812-13
Produced by FastQC (version 0.11.5)