FastQCFastQC Report
Fri 10 Feb 2017
ERR1147909.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147909.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3199979
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72790.2274702427734682No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44290.1384071582969763No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36200.11312574238768441No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31650.064.735391
GTATCAA64050.059.978781
TCAACGC84750.044.7776034
CAACGCA86850.043.6948975
ATCAACG88650.043.008983
AACGCAG90650.042.125696
TATCAAC100900.037.9165342
ACGCAGA109800.034.7786337
CGCAGAG115000.033.3094838
GTACATG120600.031.014831
GCAGAGT128750.029.7983599
TACATGG124300.029.6776432
ACATGGG128700.028.3309943
AGAGTAC113850.026.56671110-11
CATGGGG78950.024.2595984
CAGAGTA121050.024.2003310-11
TATACCG3252.3646862E-1123.7924885
GAGTACT48800.022.30545612-13
CATGGGT34200.021.566294
GTATAGG6500.021.0751911
Produced by FastQC (version 0.11.5)