FastQCFastQC Report
Fri 10 Feb 2017
ERR1147908.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147908.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4700482
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162100.3448582507070551No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92180.19610754811953326No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76810.16340877382362062No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA122500.062.3624761
GGTATCA58800.060.0496061
TCAACGC164700.045.8995744
ATCAACG166900.045.1876343
CAACGCA167500.045.1322945
AACGCAG173100.043.672216
TATCAAC177800.042.7610362
ACGCAGA204050.037.0189257
CGCAGAG211250.035.7572148
GCAGAGT233250.032.4611179
GTACATG216900.031.9815221
TACATGG224800.030.6715852
ACATGGG222400.030.4343593
TATACCG5150.027.7179245
AGAGTAC214000.027.66840710-11
CATGGGG134050.025.5571464
CAGAGTA225900.024.57846610-11
CATGGGT59200.024.5145994
GAGTACT95550.024.43237512-13
GTAAGTA23650.023.8968982
Produced by FastQC (version 0.11.5)