FastQCFastQC Report
Fri 10 Feb 2017
ERR1147907.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147907.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2863635
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106860.37316208245813454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65180.22761280679974927No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53400.1864762792744187No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT30710.10724132090856552No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38500.068.841551
GTATCAA81550.063.539921
TCAACGC109600.046.675114
ATCAACG110750.046.351583
CAACGCA113000.045.2707255
AACGCAG116900.043.8621866
TATCAAC122750.042.2696842
ACGCAGA136850.037.337557
CGCAGAG141000.036.1964238
GCAGAGT155050.032.9548159
GTACATG149800.032.0062981
TACATGG153250.031.1004032
ACATGGG152200.030.2889733
CATGGGT48400.028.0212154
AGAGTAC143850.027.43648710-11
GAGTACT58450.026.96862812-13
CATGGGG86050.026.9594634
AGTACTT62450.025.95562712-13
CAGAGTA150250.025.8521210-11
TATAACG2451.6223566E-824.2866652
Produced by FastQC (version 0.11.5)