FastQCFastQC Report
Fri 10 Feb 2017
ERR1147906.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147906.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3539595
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175700.49638447336489055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107050.3024357306414999No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82650.233501290401868No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53650.068.483121
GTATCAA111250.061.180691
TCAACGC147350.045.934454
ATCAACG150950.044.9177633
CAACGCA151700.044.617285
AACGCAG156200.043.4080436
TATCAAC160350.042.519172
ACGCAGA182350.037.183097
CGCAGAG188550.036.0235028
GCAGAGT205900.032.930249
GTACATG169050.030.2231881
TACATGG176500.028.8871842
ACATGGG174650.028.7422143
AGAGTAC191050.027.76925510-11
GAGTACT95450.027.47951312-13
CAGAGTA198350.026.40240710-11
CATGGGG93700.025.8345684
AGTACTT100700.025.48576712-13
GTACTTT103250.025.4035814-15
CATGGGT52950.023.1391644
Produced by FastQC (version 0.11.5)