FastQCFastQC Report
Fri 10 Feb 2017
ERR1147905.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147905.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2094883
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125430.5987446554294441No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79910.3814532840258859No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64270.3067951766280026No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36550.070.703221
GTATCAA77950.061.6444851
TCAACGC107600.043.943424
ATCAACG108850.043.4934273
TATCAAC110800.043.0572362
CAACGCA110500.042.8977975
AACGCAG111200.042.5742726
ACGCAGA131600.035.9746177
CGCAGAG133800.035.3831028
GTACATG126950.032.7385561
GCAGAGT145700.032.615669
TACATGG129650.031.7501012
ACATGGG128650.031.4367833
CATGGGG75250.030.03424
ATGGGGG40300.029.0736895
AGAGTAC138500.028.51394310-11
AGTACTT71900.026.88397212-13
GAGTACT68600.026.39989312-13
CAGAGTA142050.025.9800210-11
GTAAGTA15150.024.3439242
Produced by FastQC (version 0.11.5)