FastQCFastQC Report
Fri 10 Feb 2017
ERR1147904.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147904.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3653833
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183430.5020207546431378No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107530.2942936910362351No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90250.24700088920320112No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA132850.063.6508751
GGTATCA61550.061.5327951
TCAACGC182000.046.27374
ATCAACG183450.045.9413
CAACGCA186550.045.208845
AACGCAG187900.044.8207246
TATCAAC197850.042.9055522
ACGCAGA216050.038.9808587
CGCAGAG219200.038.447828
GCAGAGT236250.035.7737669
GTACATG208100.032.0496641
TACATGG212800.031.2812462
ACATGGG209100.031.2317733
CATGGGG120350.029.4044634
AGAGTAC226400.028.89739210-11
GAGTACT107500.028.21656212-13
AGTACTT111050.027.50199712-13
CAGAGTA231850.026.37110710-11
ATGGGGG57850.026.3195845
GTACTTT118550.025.38582814-15
Produced by FastQC (version 0.11.5)