FastQCFastQC Report
Fri 10 Feb 2017
ERR1147903.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147903.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2923822
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135860.46466576966723694No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79570.2721437898750334No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66500.22744202622457863No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46500.064.692261
GTATCAA95400.060.941811
TCAACGC129050.044.98414
ATCAACG131200.044.246943
CAACGCA132550.043.8411675
AACGCAG134300.043.2698976
TATCAAC144450.040.532262
ACGCAGA155600.037.384937
CGCAGAG162450.035.845148
GCAGAGT173200.033.5869
GTACATG157600.031.7873021
TACATGG161450.030.9205472
ACATGGG160700.030.3504583
AGAGTAC163400.028.174510-11
CATGGGT34400.027.6648834
CATGGGG88750.027.1427544
GAGTACT75550.027.12201912-13
CAGAGTA170200.025.94801510-11
AGTACTT77000.025.91606512-13
GTACTTT82500.024.76509914-15
Produced by FastQC (version 0.11.5)