FastQCFastQC Report
Fri 10 Feb 2017
ERR1147902.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147902.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2611935
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91400.3499321384337666No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52660.20161298041490314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46600.17841179049248929No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35400.061.39561
GTATCAA72250.059.586521
TCAACGC98800.042.9212884
ATCAACG101050.042.1421663
CAACGCA101500.042.0139275
AACGCAG102400.041.52856
TATCAAC107250.039.8283272
ACGCAGA119700.035.6258437
CGCAGAG123050.034.7526138
GTATACG1858.039933E-1032.186661
GTACATG130250.031.7727411
GCAGAGT136400.031.394849
TACATGG133250.030.672872
ACATGGG132350.030.2434483
CATGGGG79900.028.3607924
GTAAGTA20600.028.3024312
ATGGGGG47150.027.3727345
AGAGTAC128050.027.21812410-11
CAGAGTA131450.024.50067310-11
GAGTACT54850.024.39754312-13
Produced by FastQC (version 0.11.5)