FastQCFastQC Report
Fri 10 Feb 2017
ERR1147901.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147901.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2788575
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101910.36545547457034505No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60810.21806836825260215No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49250.17661350331262385No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34250.069.0166851
GTATCAA70000.061.4134251
TCAACGC93700.045.3218424
CAACGCA95100.044.4670145
ATCAACG97000.043.842063
AACGCAG98450.043.0143286
TATCAAC107650.039.9524352
ACGCAGA116250.036.3257267
CGCAGAG121800.034.621668
GTACATG117550.032.265691
GCAGAGT133850.031.7714149
TACATGG121750.031.1235712
ACATGGG122400.030.2735483
GTATAGG6300.030.2436081
AGAGTAC123300.027.47132110-11
GAGTACT61100.025.65005512-13
CAGAGTA129300.025.57555810-11
CATGGGG74500.025.387494
CATGGGT31700.024.5788144
AGTACTT63150.024.11101512-13
Produced by FastQC (version 0.11.5)