FastQCFastQC Report
Fri 10 Feb 2017
ERR1147900.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147900.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2156390
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96600.4479709143522276No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56440.2617337309113843No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46120.21387596863276123No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34550.060.99941
GTATCAA65000.060.359051
TCAACGC88900.043.8852964
ATCAACG90850.043.008813
CAACGCA92350.042.1170355
AACGCAG93100.041.841636
TATCAAC95700.040.8386232
ACGCAGA109850.035.461597
CGCAGAG112650.034.5273678
GTACATG107750.032.985831
TACATGG110600.032.055962
ACATGGG108200.031.7700233
GCAGAGT123200.031.7637799
CATGGGG67450.027.1572464
AGAGTAC115650.026.9979410-11
CATGGGT25900.026.177134
GAGTACT57350.025.71788212-13
ATGGGGG35850.025.5475065
CAGAGTA119400.025.17872810-11
AGTACTT60600.025.1237412-13
Produced by FastQC (version 0.11.5)