FastQCFastQC Report
Fri 10 Feb 2017
ERR1147899.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147899.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1022486
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77550.7584455924090892No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48370.4730627118610915No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39950.3907143960895308No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12530.12254446515649114No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51250.054.3864671
GGTATCA27850.051.7522241
TATCAAC68650.040.6426772
AACGCAG68550.040.5224536
ATCAACG68850.040.259493
TCAACGC69700.039.8538634
CAACGCA70350.039.5701835
ACGCAGA78600.035.49257
CGCAGAG80500.034.5070088
GCAGAGT86050.032.4196479
GTACATG74900.030.4548021
ACATGGG72550.030.2534753
TACATGG75600.029.9027962
CATGGGG41700.028.9565144
ATGGGGG20700.028.1606085
TATACCG1107.764281E-427.037325
AGAGTAC82850.027.03079210-11
GAGTACT39950.025.60931412-13
AGTACTT41350.025.31765612-13
CAGAGTA85000.025.01747110-11
Produced by FastQC (version 0.11.5)