FastQCFastQC Report
Fri 10 Feb 2017
ERR1147898.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147898.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3297272
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86860.2634298899211227No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54450.1651365128506232No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43080.13065346140688425No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA72050.062.6569821
GGTATCA34600.062.4834181
TCAACGC96300.046.448564
CAACGCA97900.045.7501955
ATCAACG98950.045.2647253
AACGCAG103200.043.5158966
TATCAAC113800.039.7365652
ACGCAGA121250.036.98887
CGCAGAG128500.034.9481778
GCAGAGT143750.031.1992539
GTACATG133750.030.4576631
TACATGG139950.029.123042
ACATGGG139400.028.7165973
AGAGTAC129400.027.51126510-11
CATGGGG83950.026.3574224
CAGAGTA136650.024.3105210-11
TACCGTA3052.764864E-1023.402497
GAGTACT56750.023.37328112-13
AGTACTT59300.023.27094312-13
GTACTTT61100.022.04995514-15
Produced by FastQC (version 0.11.5)