FastQCFastQC Report
Fri 10 Feb 2017
ERR1147896.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147896.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3604587
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113190.3140165572366543No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65950.18296132122764688No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56080.15557954350942285No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA96850.060.0117651
GGTATCA48200.057.3267251
TCAACGC129650.044.3618554
CAACGCA133050.043.3176275
ATCAACG133550.043.021843
AACGCAG134300.042.825876
TATCAAC145400.040.0225682
ACGCAGA158150.036.442687
CGCAGAG165550.034.8137138
GTACATG177150.032.6074451
GCAGAGT182750.031.6347689
TACATGG182100.031.5317332
ACATGGG179600.031.527273
CATGGGG106450.027.8811474
AGAGTAC168500.027.44455710-11
CATGGGT43150.026.6031024
ATGGGGG55800.025.5818985
AGTACTT75100.024.43267412-13
CAGAGTA175700.024.40727210-11
GTAAGTA19250.023.1825392
Produced by FastQC (version 0.11.5)