FastQCFastQC Report
Fri 10 Feb 2017
ERR1147894.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147894.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3569039
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112670.31568722000516103No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63900.1790397919439939No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55510.15553206339297496No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40300.062.3597871
GTATCAA85950.062.219671
TCAACGC117200.044.6629074
CAACGCA120450.043.5071955
ATCAACG121450.043.099983
AACGCAG121850.043.056136
TATCAAC134100.039.5787352
ACGCAGA142750.036.752297
CGCAGAG151250.034.72628
GTACATG156100.032.2367361
GCAGAGT164650.032.1167769
TACATGG164100.030.6389562
ACATGGG164250.029.8048133
AGAGTAC152300.028.60880310-11
CATGGGG99250.027.9884174
ATGGGGG58050.025.2072125
CAGAGTA161200.024.3171310-11
AGTACTT73200.024.21570612-13
GTAAGTA22400.022.3129562
GAGTACT68800.022.2196212-13
Produced by FastQC (version 0.11.5)