FastQCFastQC Report
Fri 10 Feb 2017
ERR1147893.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147893.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3568141
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128110.3590385021219733No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74910.2099412551241669No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61880.17342363992902748No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA95600.061.102691
GGTATCA47300.060.552651
TCAACGC127950.044.9531824
CAACGCA130650.043.933135
AACGCAG131500.043.6943866
ATCAACG131900.043.6520653
TATCAAC142350.040.6650122
ACGCAGA156600.036.8429377
CGCAGAG163100.035.155838
GCAGAGT179000.032.232429
GTACATG166850.032.2263221
TACATGG170550.031.2900522
ACATGGG169800.030.7560883
AGAGTAC166200.027.53923410-11
CATGGGG102550.026.4486454
CATGGGT40150.026.0736244
CAGAGTA172450.025.04077110-11
GAGTACT76450.024.97478712-13
AGTACTT80550.024.18354812-13
GTACTTT85950.022.35274714-15
Produced by FastQC (version 0.11.5)