FastQCFastQC Report
Fri 10 Feb 2017
ERR1147892.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147892.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1621536
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76250.47023316164426815No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47590.2934871627888619No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36840.22719199573737492No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24150.069.549731
GTATCAA51100.062.7082861
TCAACGC67800.046.6762584
ATCAACG69600.045.5545853
CAACGCA69400.045.5144355
AACGCAG71150.044.562186
TATCAAC74700.043.328412
ACGCAGA85150.037.2354557
CGCAGAG86800.036.4591068
GCAGAGT94150.033.739229
GTACATG80400.029.7065241
TACATGG82000.029.6033422
ACATGGG81950.028.5271033
AGAGTAC88800.028.0012510-11
CATGGGG48300.027.9571464
GTAAGTA9200.027.8083422
CTATACG1358.26154E-526.438184
CAGAGTA93350.026.22222910-11
GAGTACT44300.025.91598312-13
ATGGGGG24900.025.8011175
Produced by FastQC (version 0.11.5)