FastQCFastQC Report
Fri 10 Feb 2017
ERR1147891.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147891.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2598426
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110170.423987444706911No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67280.2589259805743939No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55730.21447599431348055No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA88050.058.168631
GGTATCA44700.057.290251
TCAACGC116800.043.1345374
ATCAACG119300.042.28133
CAACGCA119950.042.051375
AACGCAG119700.041.8907366
TATCAAC125650.040.4836922
ACGCAGA140050.035.9312067
CGCAGAG145750.034.4443868
GTACATG144450.033.1892971
TACATGG147450.032.279122
GCAGAGT156250.032.1297239
ACATGGG145450.031.9396553
CATGGGG87500.030.1148174
AGAGTAC145900.027.72286810-11
ATGGGGG44650.027.1764855
GAGTACT68500.025.70311212-13
AGTACTT70850.025.60616712-13
CAGAGTA150300.025.20954710-11
GTACTTT75650.023.3524314-15
Produced by FastQC (version 0.11.5)