FastQCFastQC Report
Fri 10 Feb 2017
ERR1147890.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147890.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3386159
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133720.39490171607417135No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76760.22668752412394103No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66590.19665349441653507No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45800.063.1956941
GTATCAA95300.060.554661
TCAACGC128050.044.9171034
CAACGCA129850.044.2028435
ATCAACG131000.043.9056133
AACGCAG132000.043.4378136
TATCAAC143550.040.2844052
ACGCAGA155050.036.9802747
CGCAGAG160950.035.6246768
GCAGAGT173950.033.1674589
GTACATG160650.032.8821031
TACATGG164600.031.771152
ACATGGG168000.030.3414553
AGAGTAC165550.027.64676510-11
CATGGGG96700.027.1254524
GAGTACT77700.025.83554512-13
CAGAGTA172850.025.18874710-11
CATGGGT39950.025.16144
AGTACTT82100.024.77694912-13
ATGGGGG49150.024.0821025
Produced by FastQC (version 0.11.5)