FastQCFastQC Report
Fri 10 Feb 2017
ERR1147889.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147889.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2456280
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145810.5936212483918771No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85080.3463774488250525No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75790.30855602781441854No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA103100.054.8115961
GGTATCA55950.054.5986861
AACGCAG137850.040.986636
TCAACGC138450.040.8519554
TATCAAC140400.040.2096752
CAACGCA140850.040.1980935
ATCAACG141300.039.985893
ACGCAGA159800.035.3195157
CGCAGAG164700.034.3048368
TATACCG1953.8198777E-1133.5492945
GCAGAGT176350.032.139779
GTACATG152150.031.857921
ACATGGG152000.031.380233
TACATGG155850.031.1468962
CATGGGG86350.030.3739724
AGAGTAC168500.027.24850710-11
ATGGGGG41150.026.5933675
CAGAGTA171600.025.33526210-11
CATGGGT27050.025.284594
GAGTACT83050.025.2790312-13
Produced by FastQC (version 0.11.5)