FastQCFastQC Report
Fri 10 Feb 2017
ERR1147888.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147888.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4229438
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130370.30824426318579445No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74540.17624090954873908No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61870.14628421081004142No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47700.064.177991
GTATCAA99800.062.7210851
TCAACGC133600.046.079214
CAACGCA136550.045.040165
AACGCAG139800.044.0356376
ATCAACG140650.043.8118063
TATCAAC156200.039.8832742
ACGCAGA167050.036.8167277
CGCAGAG175250.035.195888
GTACATG185350.034.3178441
TACATGG193550.032.924672
GCAGAGT191850.032.1815269
ACATGGG195700.031.8219623
AGAGTAC176150.028.19518510-11
CATGGGT50050.027.8088424
CATGGGG110150.027.7015444
CAGAGTA185350.025.03070610-11
AGTACTT83950.024.12501112-13
GAGTACT81600.024.01797512-13
GTACTTT86000.022.75456614-15
Produced by FastQC (version 0.11.5)