FastQCFastQC Report
Fri 10 Feb 2017
ERR1147887.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147887.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3542496
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107560.3036277246325754No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64990.18345821703115545No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55220.15587879280597633No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA95450.058.831111
GGTATCA48600.057.9557041
TCAACGC128850.043.11284
CAACGCA131900.042.0256965
ATCAACG132100.042.007093
AACGCAG132750.041.7566036
TATCAAC143200.039.1756972
ACGCAGA157350.035.1528057
CGCAGAG163200.033.9656228
GTACATG177850.033.7168431
TACATGG184000.032.396492
ACATGGG181350.032.0748863
GCAGAGT178600.031.17019
CATGGGG112350.030.9689584
ATGGGGG63550.027.889745
AGAGTAC165800.026.99390810-11
GTAAGTA24300.026.1954292
GAGTACT69000.024.8679812-13
CATGGGT39250.024.548174
CAGAGTA173050.024.23044210-11
Produced by FastQC (version 0.11.5)