FastQCFastQC Report
Fri 10 Feb 2017
ERR1147886.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147886.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2869086
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94520.3294428957514693No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56720.1976936208952956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47050.163989507459867No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA74350.062.1578141
GGTATCA35350.060.312671
TCAACGC99750.045.4976434
CAACGCA102250.044.3270575
ATCAACG103200.044.0342863
AACGCAG103050.043.9829446
TATCAAC112750.041.002512
ACGCAGA122750.036.9241687
CGCAGAG127350.035.5904358
GTACATG132400.033.2407951
GCAGAGT141350.032.2337079
TACATGG137450.031.816222
ACATGGG139250.031.0112083
AGAGTAC129750.028.46829810-11
CATGGGG85100.026.630094
AGTACTT60550.026.13032312-13
CAGAGTA136600.024.44985810-11
GAGTACT57350.024.37315412-13
CATGGGT30400.024.066274
ATGGGGG46450.022.2813135
Produced by FastQC (version 0.11.5)