FastQCFastQC Report
Fri 10 Feb 2017
ERR1147885.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147885.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3482334
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131620.3779648936604013No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78170.22447588312895891No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64200.1843591108721909No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48950.064.9628451
GTATCAA99150.061.561281
TCAACGC132700.045.5862274
ATCAACG136100.044.4911083
CAACGCA136500.044.3171585
AACGCAG137400.044.0268676
TATCAAC149850.040.9714552
ACGCAGA165800.036.4854777
CGCAGAG170650.035.378828
GCAGAGT184950.032.8041959
GTACATG175900.031.7551021
TACATGG179500.030.7899992
ACATGGG181900.029.8226173
AGAGTAC171650.027.06392910-11
CATGGGG98150.026.2410474
CATGGGT53850.026.0681294
CAGAGTA178700.025.89635510-11
GAGTACT73900.025.83712412-13
AGTACTT81400.023.1642612-13
GTACTTT83350.022.9077814-15
Produced by FastQC (version 0.11.5)