FastQCFastQC Report
Fri 10 Feb 2017
ERR1147884.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147884.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3111759
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157710.5068194548485278No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93780.3013729533681753No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77370.24863750695346265No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA113450.061.2114031
GGTATCA54850.061.0236171
TCAACGC151450.045.4805764
ATCAACG153250.044.946383
AACGCAG153700.044.930896
CAACGCA154500.044.6597375
TATCAAC158750.043.6259162
ACGCAGA177450.038.9508367
CGCAGAG183850.037.724338
GCAGAGT195500.035.4458859
AGAGTAC185550.028.915610-11
GTACATG168300.028.487221
TACATGG174650.027.1515732
ACATGGG171100.027.116353
GAGTACT87000.027.10881612-13
CAGAGTA190600.026.83874310-11
CATGGGG101250.026.436514
AGTACTT91250.026.26992612-13
GTACTTT95000.024.82597214-15
GTATAGG7950.023.2237781
Produced by FastQC (version 0.11.5)