FastQCFastQC Report
Fri 10 Feb 2017
ERR1147883.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147883.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3215106
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129800.40371919308414717No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78920.24546624590293448No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66420.20658727892641798No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46100.065.242091
GTATCAA92450.060.427321
TCAACGC126900.043.3118824
CAACGCA128900.042.5475655
ATCAACG129850.042.282753
AACGCAG129750.042.2229926
TATCAAC142950.038.8765562
ACGCAGA151050.036.2296267
CGCAGAG160200.034.197468
GCAGAGT172100.032.0056659
GTACATG152500.031.2433471
TACATGG158050.029.929382
ACATGGG158500.029.4607563
AGAGTAC157100.028.87090510-11
CATGGGG97300.026.2876874
CAGAGTA165850.025.60822510-11
AGTACTT79450.025.34320312-13
GAGTACT74800.025.2089312-13
ATGGGGG54550.023.5535145
GTAAGTA17000.023.450442
Produced by FastQC (version 0.11.5)