FastQCFastQC Report
Fri 10 Feb 2017
ERR1147882.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147882.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4739726
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154500.32596820997669484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88040.1857491340216713No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73560.155198844827739No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA116400.061.185361
GGTATCA56100.060.1851731
TCAACGC149200.047.3192444
CAACGCA152000.046.4475755
ATCAACG153750.045.9575843
AACGCAG156850.045.1630366
TATCAAC169250.041.969362
ACGCAGA183800.038.5085647
CGCAGAG192200.036.825578
GCAGAGT212750.033.5201159
GTACATG194600.030.2637521
TACATGG200950.029.042752
ACATGGG197950.028.845073
AGAGTAC194850.027.39617510-11
CATGGGG116800.025.9706754
GAGTACT91750.025.67113112-13
CAGAGTA204450.025.61522310-11
AGTACTT99550.023.74935212-13
GTACTTT101900.023.0265414-15
CATGGGT47000.022.399144
Produced by FastQC (version 0.11.5)