FastQCFastQC Report
Fri 10 Feb 2017
ERR1147881.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147881.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3391725
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101000.2977835762038491No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59930.17669474972174926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47440.13986983024861982No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39950.060.3808331
GTATCAA75700.056.2562871
TCAACGC97950.043.1747864
CAACGCA101250.041.7088625
ATCAACG102000.041.46053
AACGCAG105400.040.01026
TATCAAC117450.036.3744662
ACGCAGA125050.033.7706767
CGCAGAG131600.032.0898368
GTACATG136000.032.057751
TACATGG141550.030.853992
ACATGGG141150.030.1293833
GCAGAGT145550.029.2185659
AGAGTAC133900.025.09764110-11
CATGGGG80000.024.6838654
CAGAGTA141150.023.63997710-11
GAGTACT64050.022.28728512-13
CATGGGT36900.022.2442534
GTACTTT69500.020.53957614-15
CATGGGA58900.020.499624
Produced by FastQC (version 0.11.5)