FastQCFastQC Report
Fri 10 Feb 2017
ERR1147880.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147880.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3137724
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162910.5191979919202582No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91220.2907202800501255No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81780.2606347785847321No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55000.058.2522741
GTATCAA109900.057.9801561
TCAACGC148150.042.7182924
CAACGCA150800.041.967615
AACGCAG151500.041.8522226
ATCAACG153300.041.2832033
TATCAAC155950.040.8972472
ACGCAGA176450.035.8669057
CGCAGAG183400.034.5725828
GTACATG185000.033.6385961
TACATGG190700.032.602452
ACATGGG186800.032.542283
GCAGAGT198750.031.992239
CATGGGG106900.030.2131524
AGAGTAC187600.027.1244610-11
ATGGGGG55950.027.1090325
TATACCG3007.2759576E-1225.7748435
AGTACTT88300.025.76586212-13
GAGTACT86000.025.3829212-13
CATGGGT32200.024.7527184
Produced by FastQC (version 0.11.5)