FastQCFastQC Report
Fri 10 Feb 2017
ERR1147878.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147878.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3534919
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT163190.4616513136510341No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92890.26277829845606077No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77630.21960899245498977No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54050.062.580881
GTATCAA111900.062.2651141
TCAACGC147550.046.878784
ATCAACG150300.046.0606233
CAACGCA151300.045.7561875
AACGCAG155350.044.5633166
TATCAAC163450.042.7326932
ACGCAGA181750.038.188477
CGCAGAG187550.037.0074848
GCAGAGT203550.034.127749
GTACATG190750.032.84281
TACATGG194150.032.0838432
ACATGGG192150.031.8810523
CATGGGG110750.030.3954414
CATGGGT48100.028.6865774
AGAGTAC191550.027.97553610-11
GTAAGTA26550.027.562442
ATGGGGG63450.027.2770355
GAGTACT88350.025.95097212-13
CAGAGTA200250.025.69090310-11
Produced by FastQC (version 0.11.5)