FastQCFastQC Report
Fri 10 Feb 2017
ERR1147877.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147877.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3468378
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114550.3302696534230121No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66980.19311620590373946No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55850.16102627798930796No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41250.060.2051161
GTATCAA79950.057.5069271
TCAACGC104150.043.2867434
CAACGCA105500.042.7328345
ATCAACG107900.041.7829363
AACGCAG109750.041.0780336
TATCAAC121450.037.3306162
ACGCAGA131700.034.276857
CGCAGAG140350.032.1643148
GTACATG132950.029.7889121
GCAGAGT154850.029.1524819
TACATGG139650.028.162312
ACATGGG137800.027.8395183
AGAGTAC137350.026.3281210-11
GAGTACT67200.024.64908812-13
CATGGGT36900.024.0162324
CAGAGTA147550.023.7220510-11
AGTACTT72650.023.41398612-13
TATACCG4250.022.391135
GTACTTT74800.022.1048814-15
Produced by FastQC (version 0.11.5)