FastQCFastQC Report
Fri 10 Feb 2017
ERR1147876.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147876.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1813274
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76640.42266088853642636No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50700.2796047370667643No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38920.21463937606781988No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27100.067.6652151
GTATCAA53300.058.642991
TCAACGC72300.042.8623624
AACGCAG72650.042.5739946
CAACGCA73200.042.254115
ATCAACG74000.042.0384453
TATCAAC78850.039.4570432
ACGCAGA85200.036.233017
CGCAGAG88800.034.6301428
GCAGAGT95500.032.2628759
GTACATG80700.028.4034581
AGAGTAC89200.028.07332810-11
GAGTACT45500.027.5180312-13
TACATGG83100.027.3455682
CAGAGTA93250.026.40755310-11
ACATGGG84350.026.3732193
AGTACTT48300.025.6149112-13
CATGGGT22800.024.7836574
GTACTTT51250.024.31458314-15
CATGGGG44550.022.2969474
Produced by FastQC (version 0.11.5)