FastQCFastQC Report
Fri 10 Feb 2017
ERR1147875.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147875.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1425275
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79310.5564540176457176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49050.34414411253968535No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36590.25672238690778976No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28300.066.494261
GTATCAA58350.059.499211
TCAACGC80750.043.0917174
ATCAACG81550.042.668993
TATCAAC82850.042.1474952
AACGCAG82800.042.0248346
CAACGCA82750.041.9783445
ACGCAGA95700.036.4221957
CGCAGAG99950.034.932998
GCAGAGT107850.032.4293069
GTACATG93700.030.442441
TACATGG95850.029.604142
ACATGGG96000.028.9971333
CATGGGG50200.028.4372734
AGAGTAC102550.027.43506210-11
GAGTACT48600.026.55852512-13
TAATACG1401.0583521E-425.4919874
CAGAGTA106650.025.2091410-11
AGTACTT50700.024.63722412-13
TATACCG1451.343158E-424.6129535
Produced by FastQC (version 0.11.5)