FastQCFastQC Report
Fri 10 Feb 2017
ERR1147874.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147874.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2128275
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190540.8952790405375246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109930.5165215961283198No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100670.4730121812265802No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30290.14232183340968627No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28580.13428715743971056No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC24200.11370711021836932No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22530.10586037988511822No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA127550.058.072811
GGTATCA66050.056.8839041
AACGCAG169500.043.4765136
TATCAAC172050.043.1483352
TCAACGC174100.042.4986084
ATCAACG174250.042.427893
CAACGCA177400.041.640995
ACGCAGA194200.037.854927
CGCAGAG196400.037.40068
GCAGAGT208550.035.2216649
ATGGGGG64200.033.3518945
GTACATG186350.033.3263551
CATGGGG110550.032.9265174
ACATGGG187050.032.688013
TACATGG191400.032.508272
TGGGGGG52300.030.3642566
GTAAGTA27300.029.8512172
AGAGTAC206200.028.59941510-11
GAGTACT96200.028.31675512-13
AGTACTT100200.028.19544412-13
Produced by FastQC (version 0.11.5)