FastQCFastQC Report
Fri 10 Feb 2017
ERR1147872.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147872.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2449892
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120660.49251150662968No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70780.2889106948387929No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63940.26099109675038734No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA86850.061.375481
GGTATCA41450.059.3426861
TCAACGC115950.045.6014254
AACGCAG117650.044.891956
ATCAACG118250.044.8653563
CAACGCA118050.044.7398385
TATCAAC119050.044.7265242
ACGCAGA138450.038.1476177
CGCAGAG143250.036.9108928
GCAGAGT153150.034.602559
GTACATG146600.031.3635351
TACATGG153050.029.931532
ACATGGG153350.029.4378053
CATGGGG91200.028.6297194
AGAGTAC146000.027.9255710-11
ATGGGGG50950.027.7830665
GAGTACT60250.026.99900212-13
CAGAGTA149600.026.10060310-11
AGTACTT64100.025.74852612-13
ATAACGT2151.2378769E-724.8972556
Produced by FastQC (version 0.11.5)