FastQCFastQC Report
Fri 10 Feb 2017
ERR1147871.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147871.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1871363
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61620.3292787128953602No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35180.18799131969585806No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32740.17495269490740173No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50950.058.6624761
GGTATCA26050.055.539171
TCAACGC69050.042.3743484
ATCAACG69350.042.3625533
CAACGCA71150.040.9564935
AACGCAG71950.040.5011066
TATCAAC76700.038.3887752
GTACATG101050.036.8251531
TACATGG103500.035.6324582
ACATGGG104000.034.6530043
ACGCAGA85550.034.062597
CGCAGAG89750.032.4023138
CATGGGG62700.030.351774
GCAGAGT96600.030.227769
ATGGGGG36200.029.0781175
GCGTAAT850.007188531628.0183091
CATGGGT20950.027.8191384
AGAGTAC90300.026.60695810-11
ATGGGAG16400.024.6585035
TGGGGGG28800.024.572886
Produced by FastQC (version 0.11.5)