FastQCFastQC Report
Fri 10 Feb 2017
ERR1147870.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147870.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1945825
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58890.30264797707913094No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37300.19169246977503115No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29130.1497051379235029No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21400.067.640651
GTATCAA42000.057.8663561
TCAACGC56550.042.489614
ATCAACG58600.041.1057473
CAACGCA58850.040.829015
AACGCAG62750.038.386216
TATCAAC66650.036.243262
GTACATG80900.033.134411
ACGCAGA74950.032.0585377
TACATGG85200.031.4947622
CGCAGAG78650.030.4747588
ACATGGG86300.030.1172813
GCAGAGT85750.028.3676369
CATGGGG52600.027.8152734
AGAGTAC79100.025.75242210-11
ATGGGGG31350.025.6112145
TATCACG1651.1106547E-525.241522
GTATCGC2151.2379678E-724.8964846
CAGAGTA82850.023.36643410-11
GAGTACT35900.022.77939612-13
Produced by FastQC (version 0.11.5)