FastQCFastQC Report
Fri 10 Feb 2017
ERR1147869.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147869.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1577120
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65520.4154408034899057No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37370.23695089783909912No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33930.21513898752155827No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50000.057.5162541
GGTATCA25800.055.1558651
TCAACGC65600.043.06744
AACGCAG66300.042.433276
ATCAACG67050.042.2247473
CAACGCA67100.042.1932875
TATCAAC70700.040.140412
ACGCAGA77200.036.4420437
CGCAGAG80050.035.1446088
GCAGAGT88450.031.9414529
GTACATG78200.031.7499311
TACATGG79450.031.3763872
CATGGGG46400.031.2774094
ACATGGG78750.031.0420323
ATGGGGG23350.027.7650535
AGAGTAC82100.027.74690210-11
CAGAGTA85700.024.84626210-11
AGTACTT42600.024.78264412-13
GAGTACT40750.023.86431712-13
CATGGGT17400.022.5607534
Produced by FastQC (version 0.11.5)