FastQCFastQC Report
Fri 10 Feb 2017
ERR1147867.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147867.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2022915
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75490.37317435482954053No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48280.23866549014664482No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37940.18755113289485717No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26800.069.99491
GTATCAA56900.060.9117931
TCAACGC72900.046.5097474
CAACGCA74150.045.8059165
ATCAACG74400.045.5720443
AACGCAG78050.043.59336
TATCAAC83500.041.4014052
ACGCAGA90550.037.4440657
CGCAGAG95150.035.6338428
GCAGAGT103750.032.7374349
GTACATG95650.028.8884121
TACATGG98800.028.0643142
ACATGGG98300.027.6540853
AGAGTAC96650.027.32610510-11
GAGTACT44500.026.73416312-13
CAGAGTA100650.026.3287710-11
GTACTTT47950.024.8726714-15
CATGGGG59500.024.2932684
AGTACTT47300.023.51673712-13
TCTAGAC5100.023.3268683
Produced by FastQC (version 0.11.5)