FastQCFastQC Report
Fri 10 Feb 2017
ERR1147866.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147866.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5304816
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164230.30958660960153944No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95210.17947842111771642No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80070.150938317181972No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA55400.063.7449721
GTATCAA115850.061.6859441
TCAACGC149600.047.3142664
CAACGCA152350.046.4211735
ATCAACG157250.044.8990143
AACGCAG158300.044.7139246
TATCAAC178700.040.0198442
ACGCAGA184900.038.2491387
CGCAGAG193200.036.605938
GCAGAGT217550.032.5633749
GTACATG185800.030.2891521
TACATGG193750.028.5585842
ACATGGG191550.028.319753
AGAGTAC196100.027.79915810-11
CAGAGTA206400.025.69143710-11
GAGTACT104400.024.7266912-13
CATGGGG115700.024.5223314
AGTACTT111650.023.54725512-13
GTACTTT111350.023.2367714-15
AGTACAT141750.020.20458412-13
Produced by FastQC (version 0.11.5)