FastQCFastQC Report
Fri 10 Feb 2017
ERR1147865.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147865.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3584677
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174400.48651524251696876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104760.29224390370457365No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87960.2453777564896363No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63250.061.3843421
GTATCAA125050.061.2389641
TCAACGC168750.044.7947164
ATCAACG170250.044.4000473
CAACGCA172100.043.8536535
AACGCAG172600.043.6576966
TATCAAC177400.042.7553672
ACGCAGA202400.037.141687
CGCAGAG210150.035.7719578
GCAGAGT227250.033.1587229
GTACATG203550.032.5022541
TACATGG207750.031.6127362
ACATGGG204550.031.4595723
CATGGGG130500.028.8025934
GTAAGTA31250.027.9834562
ATGGGGG75750.027.8721685
AGAGTAC214850.027.50157410-11
GAGTACT98800.026.90763912-13
CAGAGTA221100.025.91719610-11
AGTACTT104350.025.90397612-13
Produced by FastQC (version 0.11.5)