FastQCFastQC Report
Fri 10 Feb 2017
ERR1147864.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147864.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3212196
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92260.2872178410034755No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51080.15901893906847528No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46570.1449786999298922No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA78000.060.3172341
GGTATCA38100.059.2411041
TCAACGC102700.045.3481034
CAACGCA104850.044.4749455
ATCAACG106000.043.9363173
AACGCAG108250.043.0230946
TATCAAC121550.038.866492
ACGCAGA126450.036.7837227
CGCAGAG133550.034.8281678
GTACATG145100.033.6554451
TACATGG149050.032.5338442
ACATGGG147950.032.1217773
GCAGAGT148350.031.4337569
CATGGGG83600.027.463054
AGAGTAC135050.027.32844710-11
CATGGGT39300.025.4263764
ATGGGGG42150.025.259415
CAGAGTA140550.024.67206410-11
GAGTACT59900.023.48400312-13
AGTACTT62150.023.3037412-13
Produced by FastQC (version 0.11.5)