FastQCFastQC Report
Fri 10 Feb 2017
ERR1147863.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147863.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3201887
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99930.3120972101763741No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58840.1837666351123572No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51360.1604054109342397No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA85450.060.2104071
GGTATCA43050.059.2025571
TCAACGC117050.043.595624
ATCAACG118950.042.7992633
CAACGCA120800.042.1930435
AACGCAG121650.041.947126
TATCAAC125000.040.9778332
ACGCAGA139850.036.4881447
CGCAGAG146400.034.8962758
GTACATG159100.034.209431
TACATGG162800.033.1077462
ACATGGG162300.032.613583
GCAGAGT161600.031.6507579
CATGGGG97350.029.8133544
AGAGTAC148700.027.45721810-11
ATGGGGG56550.026.0823125
GAGTACT62950.024.98946212-13
CAGAGTA155000.024.72966210-11
AGTACTT68450.023.93729812-13
GTACTTT68650.023.00122514-15
Produced by FastQC (version 0.11.5)