FastQCFastQC Report
Fri 10 Feb 2017
ERR1147862.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1147862.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3465362
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99550.2872715750908563No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60230.17380579575813435No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50310.14517963779830217No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38950.060.6946141
GTATCAA78400.059.5481
TCAACGC105550.043.8403854
CAACGCA108150.042.8414275
ATCAACG107900.042.775323
AACGCAG110250.041.971456
TATCAAC124900.037.1533472
ACGCAGA132700.034.870787
CGCAGAG138500.033.4963768
GTACATG146700.033.325771
TACATGG151250.032.057382
ACATGGG152150.030.999473
GCAGAGT151700.030.6993529
CATGGGG92600.027.7476234
AGAGTAC137700.027.42791710-11
GAGTACT61000.024.5710512-13
CAGAGTA144400.024.3223610-11
ATGGGGG46200.023.3018075
AGTACTT64500.023.23773812-13
GTACTTT65600.023.07474714-15
Produced by FastQC (version 0.11.5)